Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification
نویسندگان
چکیده
منابع مشابه
Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification
Microfluidic components and systems for rapid (<60 min), low-cost, convenient, field-deployable sequence-specific nucleic acid-based amplification tests (NAATs) are described. A microfluidic point-of-care (POC) diagnostics test to quantify HIV viral load from blood samples serves as a representative and instructive example to discuss the technical issues and capabilities of "lab on a chip" NAAT...
متن کاملMicrofluidic devices for nucleic acid (NA) isolation, isothermal NA amplification, and real-time detection.
Molecular (nucleic acid)-based diagnostics tests have many advantages over immunoassays, particularly with regard to sensitivity and specificity. Most on-site diagnostic tests, however, are immunoassay-based because conventional nucleic acid-based tests (NATs) require extensive sample processing, trained operators, and specialized equipment. To make NATs more convenient, especially for point-of...
متن کاملDiagnostic Devices for Isothermal Nucleic Acid Amplification
Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications...
متن کاملMicrofluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA).
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a commercially available centrifugal analyzer for i...
متن کاملAn integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids.
A self-contained, integrated, disposable, sample-to-answer, polycarbonate microfluidic cassette for nucleic acid-based detection of pathogens at the point of care was designed, constructed, and tested. The cassette comprises on-chip sample lysis, nucleic acid isolation, enzymatic amplification (polymerase chain reaction and, when needed, reverse transcription), amplicon labeling, and detection....
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ژورنال
عنوان ژورنال: Microarrays
سال: 2015
ISSN: 2076-3905
DOI: 10.3390/microarrays4040474